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Center for Vaccines and Immunology

Translational Vaccine Unit

The Antibody Core

Mission:  The Antibody Core in the Center for Vaccines and Immunology at the University of Georgia provides laboratory analyses of humoral immune responses from pre-clinical animal studies and clinical vaccine efficacy studies. 

The Octet Red384 (Forte Bio) platform includes instruments, biosensors, reagents and assay kits for analysis of biomolecular interactions in 384-well microplates. Octet systems enable real-time, label-free analysis for determination of affinity, kinetics and concentration. 

The Cellular FACS Core

The Cellular FACS Core provides services for cell sorting and phenotype acquisition using the BD FACSARIA Fusion.

The Cellular Core at the Center for Vaccines and Immunology at the University of Georgia is outfitted with a state-of-the-art Becton-Dickinson FACSAria Fusion.  

This instrument is a fully integrated advanced cell sorter enclosed in a Class II A2 biosafety cabinet; enabling users to sort BSL-2 infectious materials. In addition, the FACSAria Fusion has redundant features to further reduce the risk of operator exposure during a sort with the built-in Aerosol Management System (AMS).

This FACSAria Fusion is outfitted with 4 lasers: blue octagon laser with 3 PMTs, red octagon laser with 2 PMTs, Yellow-Green octagon laser with 4 PMTs, and a violet octagon laser with 3 PMTs. This combination of lasers allows users to create panels of up to 11 colors simultaneously. In addition, the instrument is equipped with an Automated Cell Deposition Unit (ACDU), enabling users to sort onto slides or 6, 24, 48, 96 and 384-well plates. The water recirculator bath may be used for the sample tubes as well as the collection tubes/plates to be temperature controlled from 4°C to 42°C.

The staff at the Cellular Core are available to meet and assist in project planning to ensure maximum value from your sorts and samples.

Sorting Facility

The BD FACSARIA FUSION is a flexible and highly adaptable sorter. It contains four fixed-aligned, air-cooled lasers. In its present configuration, the sorter can evaluate fifteen parameters (forward and side scatter plus eleven fluorescent channels). The FACSARIA FUSION has an increased sensitivity and flexibility due to the octagon and trigon optical arrays. This instrument is equipped with temperature-controlled chambers and has the ability to sort single cells into 96- or 384-well plates. This instrument also has an aerosol management system to eliminate the emission of aerosols generated during operation. Further, the FACSARIA FUSION is designated for sorting of infected samples and will be operated by individuals wearing respirators to further decrease the potential for contact with infectious aerosols. In addition, the entire instrument is contained in a laminar flow biosafety cabinet that is certified annually.  This instrument is operated by a qualified, designated operator and the instrument is pre-scheduled with the operator.

For more information, contact CVI at tedross@uga.edu 

The Biomarker Core

The Biomarker Core provides services to identify biomarkers for vaccine efficacy and microbial virulence.

For more information, contact CVI at tedross@uga.edu 

 

The Protein Production Core

 

The Translational Medicine Core

The Translational Medicine Core provides all epidemiological and clinical services during clinical trials.

For more information, contact CVI at tedross@uga.edu

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