What viruses do we test for?
Routine hemorrhagic disease (HD) testing is for epizootic hemorrhagic disease virus (EHDV) and bluetongue virus (BTV). SCWDS can also test for cervid adenovirus if warranted based on gross and histopathology findings.
What species do we test?
SCWDS tests all North American cervids (e.g. white-tailed and mule deer, elk, moose), pronghorn, and bighorn sheep. For other species not listed, please contact the diagnostic service or SCWDS HD laboratory for further guidance before shipment.[WU1]
What tests are run and when?
In peak HD season (June through November), samples are tested via PCR and virus isolation. Serotyping can also be performed for cases that tested positive for EHDV+ or BTV+ at other laboratories. During non-peak seasons, testing will be limited to PCR only.
What samples are preferred for testing?
Preferred samples are pieces of fresh (unfrozen) lung and/or spleen for EHDV, BTV, and cervid adenovirus. Lymph node or refrigerated whole blood in anticoagulant can also be submitted if the other two samples are unavailable. Samples of approximately 1 cm3 in size are preferred.
How should samples be stored?
Samples should be stored refrigerated and shipped with ice packs. If long delays (e.g. months) between collecting and shipping are anticipated, samples can be frozen; however, this may impact test sensitivity.
How much does testing cost?
Currently, all hemorrhagic disease testing is performed at no charge, regardless of SCWDS membership status.
Last updated February 10, 2023