The University of Georgia
College of Veterinary Medicine
Class of 2020
Developing a PIV5-based ETEC vaccine
Jazmyne Taylor, Zhuo Li, Biao He
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia
Enterotoxigenic Escherichia coli (ETEC) is one of the leading bacterial agents of diarrhea, which is the number one cause of death in children’s diarrhea under five years old. This type of infection kills thousands of people every year in developing countries and affects millions of people from developed countries as Traveler’s diarrhea. ETEC also causes outbreaks in animal populations that are devastating to these countries’ economies. Yet, there is no licensed vaccine available currently. Experimental vaccines have some limitations. Parainfluenza virus 5 (PIV5), a paramyxovirus, is not known to cause any illness in humans. Previous work has demonstrated that PIV5 is an excellent vaccine vector. In this study, two multiepitope fused antigens of ETEC WZ1 and WZ2 were inserted in PIV5 genome between the SH and HN genes. These two recombinant viruses (rPIV5-WZ1 and rPIV5-WZ2) were successfully rescued and confirmed by whole genome sequencing. One plaque-purified clone of rPIV5-WZ1 or rPIV5-WZ2 containing the exact input sequences was selected for future study. In future studies, we expect to determine WZ1 and WZ2 proteins expression in viruses-infected cells and test the immunogenicity and efficacy of rPIV5-WZ1 and rPIV5-WZ2 in animal models.
Research Grant: This work was supported by endowment from Fred C. Davison Distinguished University Chair in Veterinary Medicine to B.H.
Student Support: NIH Office of Research Infrastructure Programs, Grant Number 2T35OD010433-11