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Lea Pearlman

Lea Pearlman

The University of Georgia
College of Veterinary Medicine
Class of 2020


Research Interests

Effect of acute exposure to green tea extract and citrus fruit juice on antioxidant defenses in healthy pigs
 

Lea H. Pearlman, Xi Fang, Hea jin Park, Simon R. Platt

Department of Small Animal Medicine and Surgery (Pearlman, Platt), College of Veterinary Medicine, Department of Foods and Nutrition (Fang, Park), University of Georgia, Athens, GA

Catechins, the functional polyphenols from green tea, mitigate oxidative stress and inflammatory responses. Low oral bioavailability of catechins limit its utilization in therapeutics and lemon juice has been suggested to enhance the bioavailability. We hypothesize that lemon juice (LJ) increases catechin bioavailability and shows a synergistic effect on antioxidant activity with green tea extract (GT). Pigs (n=26, 45.563.4kg) were provided two doses of GT (190mg/kg/day) or GT plus LJ (0.75ml/kg/day) (GL) and blood samples were collected for 48 hrs using catheters placed in jugular veins. The dose of GT was selected based on literature reporting beneficial effects in humans and adjusted to pigs. A subset of blood samples were used for the current study (n=3/group, 0 and 3 hr post treatment where Vmax of blood catechins level expected). Activities of antioxidant enzymes (catalase (CAT) and superoxide dismutase (SOD)) and level of lipid peroxidation (malondialdehyde, MDA) were measured. Two pigs in GT tended to decrease in CAT activity and MDA level, whereas all pigs in GL tended to increase in activities of CAT and SOD (p > 0.05). CAT, SOD and MDA were not correlated (p > 0.05). Neither GT nor GL cause hepatotoxicity. In summary, acute administration of GT or GL did not alter antioxidant defenses or oxidative stress. The inconsistent trends observed may be due to the small subset analysis from the large scale experiment. Ongoing blood catechins analysis will provide further insight on whether LJ affects bioavailability and physiological function of GT. This study acts as a platform for larger studies evaluating beneficial activity of phytochemicals on oxidative stress and inflammation in animal disease models such as stroke.

Research Grant: The project was supported by grants from Georgia Experimental Agricultural Station (HATCH #GEO00795) and Office of the Vice President for Research at the University of Georgia (Faculty Research Grant)

Student Support: Boehringer Ingelheim, Veterinary Medical Experiment Station, UGA College of Vet Medicine

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