The University of Georgia
College of Veterinary Medicine
Class of 2019
Assessment of GI injury in Alaskan sled dogs using intestinal fatty acid binding protein and diamine oxidase
Matthew C. Tanner, Tracy Hill, Michael Davis, Robert M. Gogal, Jr.
Department of Small Animal Medicine and Surgery (Tanner, Hill) and Department of Veterinary Biosciences and
Diagnostic Imaging (Gogal), College of Veterinary Medicine, University of Georgia, Athens, GA and Center
for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma (Davis)
Background: Changes in gastrointestinal (GI) permeability and injury are caused by a breakdown of the protective gastrointestinal epithelial barrier and commonly occurs in humans, horses, and dogs following strenuous exercise. Gastrointestinal injury and increased permeability raise the risk of ulceration and translocation of intestinal bacteria causing sepsis or systemic inflammatory response syndrome (SIRS). Current methods to assess intestinal injury and permeability in dogs are either invasive, difficult to perform, or have high variability. Intestinal fatty acid binding protein (I-FABP) and diamine oxidase (DAO) are two enterocyte proteins absorbed from the epithelium with intestinal injury. Hypothesis/Objectives: We hypothesize I-FABP and DAO concentrations will be correlated with previously obtained assessments of intestinal permeability and injury. Animals: Serum was collected after various distances of a racing trial in conditioned Alaskan sled dogs. Methods: I-FABP and DAO levels will be quantified using commercially available canine-specific ELISA assays. Protein concentrations will be correlated with 5-sugar gastrointestinal permeability and severity of gastroduodenal ulceration, previously obtained by one of the authors (Davis). Results: Pending. Conclusions and Clinical Importance: This study establishes the utility of serum I-FABP and DAO in the assessment of gastrointestinal permeability and injury in dogs. Future research will continue to validate these easily quantified biomarkers and investigate their potential both as a prognostic marker as well as an assessment of therapies in diseases of gastrointestinal injury in dogs, including sepsis/SIRS, exercise, obesity, and heart disease.
Research Grant: None
Student Support: Boehringer Ingelheim, Veterinary Medical Experiment Station, UGA College of Vet Medicine