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Ryan Peiffer

Ryan Peiffer

The University of Georgia
College of Veterinary Medicine
Class of 2019


Research Interests

Transcriptomics of IgE-mediated cutaneous reactions in a canine model of atopic dermatitis
 

Ryan Peiffer, Kathleen Hoover, Frane Banovic

Department of Small Animal Surgery and Medicine, University of Georgia College of Veterinary Medicine

Atopic dermatitis (AD) is a common, chronic, recurrent, inflammatory, and pruritic allergic skin disease that develops spontaneously with nearly identical clinical phenotypes in humans and dogs. The cutaneous reactions induced by intradermal injection of anti-immunoglobulin E (IgE) antibodies in both humans and dogs grossly and histologically mimic changes seen in naturally occurring allergic dermatitis in these species. The main objective of this study was to evaluate the molecular signature of IgE-mediated cutaneous reactions using next-generation RNA sequencing (RNA-seq). Intradermal injection of saline, histamine and anticanine-IgE antibodies was performed on the left and right thorax of eight healthy male castrated Beagles. Clinical scoring was conducted at 20 min and 6 h for global wheal scores and late phase reactions, respectively. Skin biopsies were obtained for histological evaluation and RNA isolation at 6 h and 24 h from anti-IgE-associated skin reactions, and at 6 h from the saline injection site, which served as the control. For the purpose of this study we also optimized the methodology for the extraction of RNA from small skin biopsies of suitable quality for sequencing by comparing the collection and storage of skin tissues in RNAlater to immediate snap freezing. Extracted RNA was subjected to both RNA-seq and real-time PCR for genes of interest. Comparative transcriptome analysis of canine IgE-mediated skin reactions will reveal differences in gene expression related to epidermal keratinocyte differentiation, innate and adaptive immune responses, and pruritogenic pathways. Our observations will allow for molecular comparison of the IgE-mediated model to natural human and canine AD.

Research Grant: Merial-Boehringer Ingelheim

Student Support: Boehringer Ingelheim, Veterinary Medical Experiment Station, UGA College of Vet Medicine

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