Viral cloning of weak PCR products for sequencing

Department of Population Health, PDRC Diagnostic Services and Teaching Laboratory, Poultry Diagnostic and Research Center

Test Code: VRCL

Species: N/A

Preferred Specimen

PCR or RT-PCR product

Acceptable Specimen

Not Applicable

Collection Protocol

PCR or RT-PCR products that are too weak to sequence may be cloned to obtain sufficient amount of product for sequencingPCR or RT-PCR products that contain mixed populations of viruses may be cloned to obtain sequences

Section

Virology

Method

Cloning product into PCR vector and transforming E. coli
Clones will be submitted for Sanger sequencing

Test Days and Turnaround

Performed when needed; Up to 3 weeks

Shipping Requirements

Not Applicable

Ship To Address

N/A

Contact Information

For any questions regarding prices, sample collection, testing, shipping requirements, and permits please call (706) 542-5657 or email [email protected]. Prices are subject to change without notice.

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