The Octet® system provides researchers the opportunity to perform high – throughput, real – time , drug – protein and protein – protein binding kinetics in an expedited manner compared to traditional techniques.

The high – throughput aspect of the system means that a library of small molecules or known proteins can be screened for interaction with a protein of interest both quickly and at large scale. The BioLayer Interferometry (BLI) Technology provides real – time protein – small molecule or protein – protein association and dissociation kinetics. The high sensitivity of the assay means that much lower protein amounts are required for analysis compared to traditional immunoprecipitation, NMR, or ELISA methods. In addition, crude protein extracts may be used, and the OctetÒ system has considerable flexibility to work with both labeled and non – labeled protein targets.

ForteBio’s Octet® platform includes instruments, biosensors, reagents and assay kits for performing label-free, real-time protein quantitation and biomolecular binding kinetic analysis. in 96- or 384-well microplates.

• Biomolecular Kinetics: The Octet accurately measures kinetic constants by bringing the detection surface directly to the sample, eliminating the need for micro-fluidics. This approach utilizing label-free, real-time analysis allows direct measurement of crude samples.
• Affinity Measurements — ka, kd, KD : Real-time kinetic measurements offer a direct realistic depiction of molecular interactions. The Octet-384 is label-free system for full affinity determination, enabling users to easily and accurately obtain kinetic constants such as ka, kd, KD.
• Kinetic Screening and Clone Selection: Researchers looking to select the best clones from a large pool will reap substantial benefits with the Octet due to its throughput and crude sample compatibility.
• Epitope Binning: When multiple candidates have similar affinities for a target, determining their preference for the same or different epitopes on the target molecule becomes a crucial factor in their evaluation. Separating the candidates into groups (bins) with a common preference for the same epitope helps researchers further characterize the specificity of the clones and their ability to block target activity.
• Ligand Capture: With a wide range of ligand capture surfaces that allow for the immobilization of antibodies, recombinant proteins, DNA, RNA and virus like particles (VLPs), ForteBio’s Octet 384 provides real-time measurements in the analysis of therapeutic biological molecules at different stages of development. The applications include but are not limited to protein:protein, protein:nucleic acids, protein:small molecules interactions and the analysis of large molecules such as virus-like particles.

The following sensors are available.

• Anti-GST (GST). For Quantitation and Kinetic Characterization of GST-tagged Biomolecules
• Aminopropylsilane (APS) Biosensors. For Hydrophobic Immobilization of Proteins
• Anti-Penta-His (HIS1K). For Label-Free Quantitation and Kinetic Analysis of His-Tagged Recombinant Proteins
• Anti-hIgG FC Capture (AHC). For Label-Free Immobilization and Subsequent
• Kinetic Characterization of Human IgG or other Human Fc-containing Proteins
• Anti-mouse IgG FC capture (AMC). For Immobilization and Subsequent Kinetic
• Characterization of Mouse IgGs and other Fc-Containing Proteins
• Protein A (ProA). For Determination of Antibody Concentration
• Protein G (ProG). For Determination of Antibody Concentration
• Streptavidin (SA). For Kinetic Analysis, Screening, and Quantitation of Most Biotinylated Proteins

*If CVI Core Director performs analysis

For information on scheduling the Octet or the Bio-Plex, contact the CVI Antibody Core Manager (Michael Kulik; [email protected]; ext. 2-6012).

Our instrument: FACSAria Fusion

Equipped with 4 lasers (Violet, Blue, Yellow, and Red) and exclusively operated by the Flow Core staff.

• Up to 13 colors sorting for immunophenotyping
• Sorting and analysis of CFP, GFP, YFP, mRFP, mTomato, CFP and mCherry gene expression proteins
• DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.)
• Sorting stem cell side populations
• Sorting into 15ml falcon, 5ml FACS tubes and Eppendorf or single cell sorting into 6, 24, 48, 96, or 384-well plates for culture and slides
• Biohazardous cell sorting for BSL2 infected samples

Researchers interested in using the FACSARIA Fusion should complete a request form below and submit to Celia Saney ([email protected]) to schedule a consultation.

CVI Cellular Core Sort Request Form