Center for Vaccines and Immunology, Department of Infectious Diseases, Department of Population Health, Poultry Diagnostic and Research Center
Associate Professor, CVI Affiliated Faculty, Joint Faculty
Biography
Research Interests
The focus of our laboratory is the characterization of enveloped virus entry. Specifically we are interested in the mechanism of virus-cellular fusion utilizing arenaviruses as our model. We perform structure-functional analyses on the Old World arenavirus glycoprotein complex. As we define the functional micro-domains within the protein, we hope to provide new targets for therapeutics and vaccine development.
Educational Background
BS (2002), Microbiology, Arizona State
PhD (2007), Microbiology, University of Iowa
Activities
- 13th Southeastern Regional Virology Conference (April 2014), Efficient Entry of a Paramyxovirus Does Not Require Fusion Protein Six-Helix Bundle Closure, (oral presentation), 2014
- American Society of Virology meeting (June 2014), Efficient replication of a paramyxovirus independent of full zippering of the fusion protein six-helix bundle domain, (oral presentation), 2014
- American Society of Virology (oral presentation), 2012
- Southeastern Regional Virology Conference (oral presentation), 2012
- American Society of Virology (oral presentation), 2011
- Paramyxer (oral presentation), 2010
- American Society of Virology (oral presentation), 2010
- Southeastern Regional Virology Conference (oral presentation), 2010
- American Society of Virology (oral presentation), 2009
- Paramyxer (oral presentation), 2008
- Keystone – Cell Biology of Virus Entry (poster), 2008
- Conference on Retroviruses and Opportunistic Infections (poster), 2008
Selected Publications
Brindley MA*, Chaudhury S*, Plemper RK. Measles Virus Glycoprotein Complexes Preassemble Intracellularly and Relax during Transport to the Cell Surface in Preparation for Fusion. J Virol. 2015 Jan 15;89(2):1230-41. PMID: 25392208 (*authors contributed equally)
Brindley MA, Plattet P, Plemper RK. Efficient replication of a paramyxovirus independent of full zippering of the fusion protein six-helix bundle domain. Proc Natl Acad Sci U S A. 2014 Sep 9;111(36):E3795-804. PMID: 25157143
Kiss G, Chen X, Brindley MA, Campbell P, Afonso CL, Ke Z, Holl JM, Guerrero-Ferreira RC, Byrd-Leotis LA, Steel J, Steinhauer DA, Plemper RK, Kelly DF, Spearman PW, Wright ER. Capturing enveloped viruses on affinity grids for downstream cryo-electron microscopy applications. Microsc Microanal. 2014 Feb;20(1):164-74. PMID: 24279992
Brindley MA*, Suter R*, Schestak I, Kiss G, Wright E, Plemper RK. A stabilized headless measles virus attachment protein stalk efficiently triggers membrane fusion. J Virol. 2013 Aug 21. PMID: 23966411 (*authors contributed equally)
Tahara M, Ohno S, Sakai K, Ito Y, Fukuhara H, Komase K, Brindley M, Rota P, Plemper R, Maenaka K, and Takeda M. The receptor-binding site of the measles virus hemagglutinin protein itself constitutes a conserved neutralizing epitope. J Virol. 2013 Mar;87(6):3583-6. PMID: 23283964
Tahara M, Ito Y, Brindley MA, Ma X, He J, Xu S, Fukuhara H, Sakai K, Komase K, Rota PA, Plemper RK, Maenaka K, Takeda M. Functional and structural characterization of neutralizing epitopes of measles virus hemagglutinin protein. J Virol. 2013 Jan; 87(1):666-75. PMID: 23115278
Ader N, Brindley M, Avila M, Orvell C, Horvat B, Hiltensperger G, Schneider-Schaulies J, Vandevelde M, Zurbriggen A, Plemper RK, Plattet P. Active membrane fusion triggering mechanism by morbillivirus attachment protein. J Virol. 2013 Jan;87(1):314-26. PMID: 23077316
Brindley MA, Takeda M, Plattet P, Plemper RK. Triggering the measles virus membrane fusion machinery. Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):E3018-27. PMID: 23027974
Ader N, Brindley MA, Avila M, Origgi FC, Langedijk JP, Orvell C, Vandevelde M, Zurbriggen A, Plemper RK, Plattet P. Structural rearrangements of the central region of the morbillivirus attachment protein stalk domain trigger F protein refolding for membrane fusion. J Biol Chem. 2012 May 11;287(20):16324-34. PMID: 22431728
Plemper RK, Brindley MA, Iorio RM. Structural and mechanistic studies of measles virus illuminate paramyxovirus entry. PLoS Pathog. 2011 Jun;7(6). PMID: 21655106 review
Kondratowicz AS, Lennemann NJ, Sinn PL, Davey RA, Hunt CL, Moller-Tank S, Meyerholz DK, Rennert P, Mullins RF, Brindley M, Sandersfeld LM, Quinn K, Weller M, McCray PB Jr, Chiorini J, Maury W. From the Cover: T-cell immunoglobulin and mucin domain 1 (TIM-1) is a receptor for Zaire Ebolavirus and Lake Victoria Marburgvirus. Proc Natl Acad Sci U S A. 2011 May 17;108(20):8426-31. PMID: 21536871 Featured article.
Brindley MA*, Hunt CL*, Kondratowicz AS, Bowman J, Sinn PL, McCray PB Jr, Quinn K, Weller ML, Chiorini JA, Maury W. Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein. Virology. 2011 Jul 5;415(2):83-94. PMID: 21529875 (*authors contributed equally)
Oh C, Price J, Brindley MA, Widrlechner MP, Qu L, McCoy JA, Murphy P, Hauck C, Maury W. Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L. Virol J. 2011 Apr 23;8:188. PMID: 21513560
Brindley MA and Plemper RK. Blue Native PAGE and Biomolecular Complementation Reveal a Tetrameric or Higher-Order Oligomer Organization of the Physiological Measles Virus Attachment Protein H. J Virol. 2010 Dec; 84(23):12174-84. PMID: 20861270 Featured article.
