Biography

Nicolle Barbieri

Department of Population Health, Poultry Diagnostic and Research Center
BS, MS, PhD
Assistant Professor

Biography

Dr. Barbieri is an assistant professor in the Department of Population Health at the University of Georgia College of Veterinary Medicine. She is specialized in Extra-intestinal pathogenic E. coli (ExPEC) – which encompass avian pathogenic Escherichia coli (APEC), Uropathogenic E. coli (UPEC) and Neonatal meningitis E. coli (NMEC). She has been studying their characteristics, looking for the prevalence of virulence genes, drug resistance, and their role in pathogenesis by using molecular biology tools to elucidate their relationship with their host. Currently, Dr. Barbieri research focuses are on antimicrobial resistance and virulence factors in ExPEC of poultry isolates (broilers, turkeys and layers) and onto investigating the molecular basis for biofilm formation as well as their relationship with human pathogens.

Research Interests

Dr Barbieri is an assistant professor in the Department of Population Health at University of Georgia College of Veterinary Medicine. She is specialize in Extra-intestinal pathogenic E. coli (ExPEC) – which encompass avian pathogenic Escherichia coli (APEC), Uropathogenic E. coli (UPEC) and Neonatal meningitis E. coli (NMEC). She has been studying their characteristics, looking for the prevalence of virulence genes, drug resistance and their role in pathogenesis by using molecular biology tools to elucidate their relationship with their host. Currently, Dr Barbieri research focuses are on antimicrobial resistance and virulence factors in ExPEC of poultry isolates (broilers, turkeys and layers) and investigate the molecular basis for biofilm formation as well as their relationship with human pathogens.

Interests:

– Poultry microbiology and foodborne pathogens

– Extra-intestinal pathogenic E. coli prevalence of virulence genes

– Colistin resistance (mcr-1 to mcr -8) in Escherichia coli.

– APEC prevalence of virulence factors and antimicrobial resistance

– APEC molecular diagnostics

– APEC host-bacteria interaction

– APEC biofilm formation

Educational Background

PhD (2014)        Universidade Federal do Rio Grande do Sul, Brazil. Ph.D in Cell and Molecular Biology

MS (2010)         Universidade Federal do Rio Grande do Sul, Brazil.  Master in Cell and Molecular Biology

BS (2007)         Universidade Federal do Rio Grande do Sul, Brazil, Graduation in Pharmacy

Selected Publications

N. L. Barbieri, J. A. Vande Vorde, A. L. Baker, F. Horn, G. Li, C. M. Logue, L. K. Nolan (2017) FNR Regulates the Expression of Important Virulence Factors Contributing to the Pathogenicity of Avian Pathogenic Escherichia coli. Frontiers in Cellular and Infection Microbiology June 2017

N. L. Barbieri; D.W Nielsen; Y. Wannemuehler; T. Cavender; A. Hussein; S.Yan; L. K. Nolan; C. M Logue (2017) mcr-1 identified in Avian Pathogenic Escherichia coli (APEC) PLOS one Accepted Febuary 2017.
C. M. Logue, Y. Wannemuehler, B. A. Nicholson, C. Doetkott, N. L. Barbieri, L. K. Nolan (2017) Comparative Analysis of Phylogenetic Assignment of Human and Avian ExPEC and Fecal Commensal Escherichia coli Using the (Previous and Revised) Clermont Phylogenetic Typing Methods and its Impact on Avian Pathogenic Escherichia coli (APEC) Classification Frontiers in Microbiology Accepted January 2017. 8(283)

Nicholson, B., West, A.C., Mangiamele, P., Barbieri, N.L., Wannemuehler, Y., Nolan, L.K., Logue, C.M. and Li, G. (2015) Genetic Characterization of ExPEC-like Virulence Plasmids among a Subset of NMEC Frontiers in Microbiology Jan 22, 2016. 0147757

Nicholson, B., West, A.C., Mangiamele, P., Barbieri, N.L., Wannemuehler, Y., Nolan, L.K., Logue, C.M. and Li, G. (2016) Large Virulence Plasmids in Neonatal Meningitis Escherichia coli: Phylogenetic Relationships and Core and Pan-Genome Plos One. 2016, Jan 22 (1), p.e0147757.

Oliveira, A. L., Rocha, D. A., Finkler, F, Moraes L, Barbieri, N.L., Pavanelo, D. B., Winkler, C., Brito, K., Brito, B. G., Horn, F. (2015) Prevalence of ColV plasmid-linked genes and in vivo pathogenicity of avian strains of Escherichia coli. Foodborne Pathogens and Disease. Accepted April 17, 2015. 12:8:679-685

Barbieri, N.L., Oliveria, A.L., Tejkowski, T.M., Pavanelo, D.B., Metter, L. B., Schincariol Pinheiro, S.R., Ibelli Vaz, T.M., Guimaraes de Brito, B., Nolan, L.K., Logue, C.M., and Horn, F. (2015) Molecular Characterization and Clonal Relationships Among Escherichia coli Strains Isolated from Broiler Chickens with Septicemia. Fodborne Pathogens and Disease Accepted 09/14 12:1:74-83

Barbieri, N.L., Nicholson, B., Hussein, A., Cai, W., Wannemuehler, Y., Dell’Anna, G., Logue, C.M., Horn, F., Nolan. L.K., and Li, G. (2014) FNR Regulates the Expression of Important Virulence Factors Contributing to the Pathogenicity of Uropathogenic Escherichia coli. Infection and Immunity Accepted 09/14 82:12:5086-5098

Cai W, Wannemuehler Y, Dell’anna G, Nicholson B, Barbieri, N.L., Kariyawasam S, Feng Y, Logue CM, Nolan LK, Li G. (2013) A Novel Two-Component Signaling System Facilitates Uropathogenic Escherichia coli’s Ability to Exploit Abundant Host Metabolites. PLoS Pathogens (Online) , v.9, p.e1003428 – , 2013.

Barbieri, N.L., Oliveira, A. L., Tejkowski, T. M., Pavanelo, D.B., Rocha, D. A., Matter, L. B., Callegari-Jaques, S. M., Brito, B. G., Horn, F. (2013) Genotypes and Pathogenicity of Cellulitis Isolates Reveal Traits That Modulate APEC Virulence. Plos One. v.8, p.e72322 – , 2013.

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