From Neural Stem Cell to Myelin...

 Vision: Create a world where every myelin disease has a therapy.

Mission: Discover and pioneer innovative stem cell therapies that will transform the course or cure myelin disorders.

Strategy: Discover the molecular and cellular mechanisms regulating neural stem cells in myelin diseases.

The myelin sheath is a specialized membrane synthesized by oligodendrocytes, which wraps around the axons of neurons in the vertebrate brain. Myelin is lost in normal aging as well as in several neurological diseases which include Multiple Sclerosis, Alzheimer’s disease, and Schizophrenia. In demyelinating diseases, disruption of myelin results in severe neurological deficits due to conduction block leading to the loss of axons and ultimately neurodegeneration. The goal in treating neurological diseases with myelin loss is to ensheathe the demyelinated axons before the neurons degenerate.

Our lab focuses on how neural stem cells regenerate myelin (remyelinate) following demyelination. Our objective is to understand the molecular mechanisms underlying  proliferation, migration and differentiation of neural stem cells into myelinating oligodendrocytes to help develop  therapeutic strategies for remyelination.

Live imaging of primary adult neural stem cells showing migration and cell division of Gli1 neural stem cell (red) along with neural stem cells infected with GFP-lentivirus (green)

SELECTED PUBLICATIONS

*Radecki DZ, Wang AR, Johnson AS, Overman CA, Thatcher MM, Iyer G, Samanta J. Gpnmb inhibits oligodendrocyte differentiation of neural stem cells by amplifying TGFβ1 signaling. bioRxiv 2021.08.13.456269, in revision Nature Communications.

*Doss GA, Radecki DZ, Kethiredy A, Reilly MJ, Pohly AE, August BK, Duncan ID, Samanta J (2023). Wobbly Hedgehog Syndrome- a progressive neurodegenerative disease, Experimental Neurology, PMID:37634698

*Clawson ED, Radecki DZ, Samanta J (2023). Immunofluorescence assay for demyelination, remyelination, and proliferation in an acute cuprizone mouse STAR Protocols, PMID: 36853716

*Radecki DZ, Samanta J (2022). Endogenous neural stem cell mediated oligodendrogenesis in the adult mammalian brain. Invited review, Cells, PMID: 35805185

*Radecki DZ, Samanta J (2022). Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation STAR Protocols, PMID: 35146452

*Samanta J, Silva HM, Lafaille JJ, Salzer JL* (2021). Transcriptomic analysis of loss of Gli1 in neural stem cells responding to demyelination in the mouse brain. Scientific Data, PMID: 34711861

*Radecki DZ, Messling H, Haggerty-Skeans JR, Bhamidipati SK, Clawson ED, Overman CA, Thatcher MM, Salzer JL, Samanta J (2020). Relative levels of Gli1 and Gli2 determine the response of ventral neural stem cells to demyelination, Stem Cell Reports, PMID: 33125874

*Samanta J, Grund EM, Silva HM, Lafaille JJ, Fishell G, Salzer JL (2015). Inhibition of Gli1 mobilizes endogenous neural stem cells for Nature, PMID: 26416758

Team Myelin

 

Drew Cribbs, PhD student (8/2024 – ), CBMS program, UGA

 

 

Zane Moore, DVM student, GVSP 2024

 

Annamaria Scalia, Lab Technician

 

Jordyn Cohen, Undergraduate student

 

Jeffrey Duodu, Undergraduate student

 

Diya Lakwani, Undergraduate student

Nadia Janousek, Undergraduate Student

 

 

 

 

 

Alumni

 

Graduate Students

 

Elizabeth Clawson, PhD Student (2/2020 – 5/2024), UW-Madison

Sai Krishna Bhamidipati, MS student (9/2019 – 5/2021), UW-Madison
Currently: PhD student at UCSD

 

Post-doctoral Fellows

Daniel Radecki, Postdoctoral Fellow (3/2018 – 6/2023)
Currently: Program Officer, Additional Ventures

Contact

Jayshree Samanta, MBBS, PhD

Associate Professor

Email: jayshree.samanta (at) uga.edu

Office: 3007A College of Veterinary Medicine, 501 D. W. Brooks Drive, Athens, GA 30602

Lab: 3007 College of Veterinary Medicine, 501 D. W. Brooks Drive, Athens, GA 30602

Department of Biomedical SciencesDepartment of Physiology and Pharmacology

Center for Neurological Disease Research

Regenerative Bioscience Center

Institute of Gerontology

 

 

 

We’re UGA Vet Med, and our

passion powers our commitment.