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We utilize flow cytometry to determine the immunophenotype of cells by recognizing normal and abnormal expression of cell surface markers on T cells (helper T cells, cytotoxic T cells), B cells, neutrophils and monocytes. Flow cytometry is available for dogs and cats.
Clinical flow cytometry is most often used:
- To help distinguish between reactive and neoplastic lymphocytes
- To determine if lymphoma / leukemia is of B or T cell origin and in some cases, identify specific subtypes of B or T cell lymphoma / leukemia which will aid in prognosis
- To differentiate between lymphoid and non-lymphoid (myeloid / monocytic) neoplasia
Clinical flow cytometry is used most often in combination with clinical history/presentation and cytology / blood smear examination for diagnosis of disease. In some cases, additional diagnostics (PARR- PCR for antigen receptor rearrangement, histopathology, immunohistochemistry, immunocytochemistry) are also required.